As I mentioned on the list, this is a mpeg-2 file wrapped in a .mov wrapper.

This is for playback on an Omneon broadcast server.

It plays back fine, except for it having one black frame at the end. 

The source and destination files have the exact same number of frames, so I'm lost as to where the black is coming from.

The destination file is full HD resolution (1920x1080) - but this sample file is 720x480 so that it will fit the size requirements.

Here's the last string I tried:
ffmpeg -i input.mov -f mov -vcodec mpeg2video -qscale 1 output.mov

Thanks!